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KMID : 0903619860270020174
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Journal of the Korean Society for Horticultural Science
1986 Volume.27 No. 2 p.174 ~ p.180
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Studies on Rhizome Growth and Organogenesis of Cymbidium kanran Cultured in vitro
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김영진/Kim, Young Jin
이정식/이종석/심경구/유미선/Lee, Jeong Sik/Lee, Jong Suk/Shim, Kyung Ku/Yoo, Mi Seon
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Abstract
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This study was carried out to accelerate the growth of rhizome and shoot formation of Cymbidium kanran. Rhizome used as materials were prepared by seed sterile culture, and rhizome-tips were placed on the basal (Murashige & Skoog) medium which was supplemented with growth substances.
The results obtained were summarized as follows. Shoot formation at the rhizome-tips was best in the media supplemented with 1.0㎎/ℓ N6-benzyladenine (BA). Rhizome growth was stimulated in the media supplemented with 1.0㎎/ℓ BE1 and 1.0㎎/ℓnaphthalene acetic acid(NAA). When organic matters were added to basal medium, a noticeable promotion of rhizome growth was observed in the medium containing activated charcoal. Apple juice and banana juice exhibited growth stimulating effects too. Prominent shoot formation was achieved on the culture media containing 2 to 5% sucrose and 5.0 ㎎/ℓ BA. When 2 to 5% surcrose was applied to the basal medium, a considerable growth of rhizome was observed. Growth of rhizome was better under dark than under 16-hour-illuminated condition, and the rhizome cultured in dark was more effective for shoot formation.
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